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Selection of fluorescence filter sets
Choosing the appropriate filter set is a very important step before ordering your filters. It is essential to match the spectral characteristics of excitation and emission filters and dichroic mirror with the fluorophores used in your fluorescence microscopy application
The most important characteristics for the selection of fluorescence filter sets are:
• The separation between excitation and emission wavelengths
• The slope of the dichroic mirror
• The optical density of the emission filters
• The type of emission filter: longpass or bandpass in terms
of color discrimination
• The transmission percentage of the excitation and emission filters
• The type of coating applied to the filters in terms of durability
Standard fluorescence filter sets
Our standard fluorescence filter sets consists out of standard sputtered bandpass filter sets, high sharp slope dichroic mirrors for excellent separation and emission filters with
a high transmission level above 90% . These filter sets are offered at an affordable price and have a high hardness and excellent durability These filter sets are perfect for routine fluorescence and for most applications requiring precise color discrimination and excellent out-of-band blocking. For applications requiring the highest possible signal intensities, optimized and optimized premium filter sets are recommended
Optimized and optimized premium fluorescence filter sets
These filters sets feature the highest throughput sputter coatings with maximum transmission levels. The filters have very steep transitions and very high optical density suitable for modern fluorophores with short stokes shifts (difference
between absorption and emission maxima) and very little spectral separation between excitation and emission bands. The high hardness and excellent durability of the coatings enables these fluorescence filter sets to be used in high heat and humidity
6-position fluorescence rotating attachment
The 6-position rotating attachment can be supplied with standard fluorescence filters for routine applications for blue, green, violet and ultra-violet excitation or advanced fluorescence filters optimized for specific fluorophore(s
Standard fluorescence filter sets
Needs empty filter block
Filter set for violet excitation, DX.9747
EX: 400-410 nm Bp, Dm: 455 nm, Em: 460 nm LP
